Cellulose-fueled Single-chamber Microbial Fuel Cells Using Cellulomonas fimi

セルロースを燃料としたCellulomonas fimiを用いたシングルチャンバー微生物燃料電池

2019-09-25

2019-09-25

The purpose of this study is the development of a single-chamber microbial fuel cells (MFC) utilizing cellulose as fuel for Cellulomonas spp. with/without addition of electron mediator. Herein, the direct electron transfer of Cellulomonas fimi was studied and revealed. Celluloses of palm kernel shell was synthesized and utilized as fuel for studying electric generation for alternative renewable energy resources. In chapter 1, the principle of MFC using metabolism of microorganism to generate electron along with organic degradation. The thesis mentioned the advantage of MFC compared with thermal power generation of Carnot cycle having limitation, while MFC has not such limitation for converting chemical bond energy of organic substrate to electric energy. In chapter 2, Cellulomonas fimi, Cellulomonas biazotea, Cellulomonas flavigena were chosen to compare cellulose degradation and current generation. Cellulomonas fimi degraded cellulose with the highest quantity among the three microorganisms. The generated electric increased with cellulose degradation and the maximum electric power was 63% of the electric power generated by MFC with the well-known electric generating Shewanella oneidensis MR-1. Cellulomonas fimi was an excellent candidate to produce the electric current from cellulose via MFCs. In chapter 3, A single chamber microbial fuel cell utilizing cellulose as fuel for Cellulosemonas fimi without electron mediator provided electric generation. Cyclic voltammogram showed the oxidation peak caused by the electron transfer of cytochrome c and disappeared after adding antimycin A inhibiting electron transfer activity. This was thought that cytochrome c was on the membrane surface of the living cell and played a role in direct electron transfer. In chapter 4, Celluloses with different characterization were synthesized from palm kernel shell, and then used as fuel for Cellulomonas fimi in the MFCs to study and compare the MFC performances with commercially available cellulose. In chapter 5, general review and future perspective were described.

博士(工学)

京都工芸繊維大学

14303甲第940号

http://hdl.handle.net/10212/2550

eng

ETD

10

Thesis or Dissertation

Thesis