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Date of Approval：2019.09.25

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Number of Access this item:691（2024-05-18 15:52 Counts）

Identifier to cite or link to this item:http://hdl.handle.net/10212/2543

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title	Function of lipid storage droplet-1 and 2 in development of Drosophila melanogaster
title.alternative	ショウジョウバエlipid storage droplet-1および 2遺伝子の発生過程における機能
contributor.author	TRAN DUY BINH
subject	Drosophila
	Lipid storage droplet-1
	Lipid storage droplet-2
	Development
	Development
	dFoxO
	JNK signaling pathway
	LSD-1 may positively control JNK signaling pathway
	which is required for thorax development
	Role of LSD-2 in the development of Drosophila wings via genetically interaction with dFoxO
dateofgranted	2019-09-25
degreename	博士(学術)
grantor	京都工芸繊維大学
grantid	14303甲第933号
Category	Date of Approval：2019.09.25

title	Function of lipid storage droplet-1 and 2 in development of Drosophila melanogaster
title.alternative	ショウジョウバエlipid storage droplet-1および 2遺伝子の発生過程における機能
contributor.author	TRAN DUY BINH 著者名の異表記形チャンドゥイビン
subject	Drosophila
	Lipid storage droplet-1
	Lipid storage droplet-2
	Development
	Development
	dFoxO
	JNK signaling pathway
	LSD-1 may positively control JNK signaling pathway
	which is required for thorax development
	Role of LSD-2 in the development of Drosophila wings via genetically interaction with dFoxO
dateofgranted	2019-09-25
date.issued	2019-09-25
description.abstract	Lipid droplets comprise neutral lipids that are enclosed by a monolayer of phospholipids. The surface of a lipid droplet is decorated with several types of proteins such as structural proteins, lipid-synthesis enzymes, lipases, and membrane-trafficking proteins. Notably, Drosophila and mammals share these lipid metabolism-related genes and basic metabolism function. Perilipins are known to be involved in regulating the accessibility of lipases; e.g., mammalian adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), by either recruiting these enzymes or preventing their access to lipid droplets. Perilipins regulate ATGL activation through the interaction with comparative gene identification 58 (CGI-58), which is an ATGL coactivator. Also, the role of perilipin 1 locus was identified as obesity risk factors, and perilipin 2 participates in the pathogenesis of diet-induced insulin resistance. Drosophila encodes two perilipin family member, Lsd-1/perilipin-1 and Lsd-2/perilipin-2. LSD-1 indiscriminately labels lipid droplets of varying size whereas LSD-2 localizes to smaller lipid droplets. LSD-1 exclusively locates on lipid droplets while LSD-2 presents both in the cytoplasm and on lipid droplets. However, the roles of these genes in development remain unclear. This present study, we found that LSD-1 expresses not only in wing pouch but also detection in notum of wing imaginal disc. Specific knockdown of Lsd-1 by pannir-GAL4 driver in wing disc leads to split thorax phenotype, suggesting that an essential role of Lsd-1 in development of Drosophila thorax. This role of Lsd-1 is dependent on the activity of the Drosophila c-Jun N-terminal kinase, as overexpression of basket suppresses split thorax induced by Lsd-1 knockdown. Besides these events, depletion of Lsd-1 causes an enhancement apoptosis cell death in wing notum. Moreover, we also demonstrated that knockdown of Lsd-2 led to dramatic cell death and developmental defects in the wing blade. Also, Lsd-2 knockdown-induced apoptosis cell death was shown to be mediated by dFoxO. Taken together, these data indicate Lsd-1 and Lsd-2 have main role in Drosophila development.
degreename	博士(学術)
grantor	京都工芸繊維大学
grantid	14303甲第933号
identifier.uri	http://hdl.handle.net/10212/2543
language.iso639-2	eng
rights.textversion	none
sortkey	3
type.nii	Thesis or Dissertation
type	Thesis

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